%0 Journal Article %J Biosensors %D 2023 %T SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering %A Benešová, Markéta %A Bernatová, Silvie %A Mika, Filip %A Pokorná, Zuzana %A Ježek, Jan %A Šiler, Martin %A Samek, Ota %A Růžička, Filip %A Rebrošová, Katarína %A Zemánek, Pavel %A Pilát, Zdeněk %K BF %K MF %B Biosensors %V 13 %P 182 %G eng %U https://www.mdpi.com/2079-6374/13/2/182 %R 10.3390/bios13020182 %0 Journal Article %J FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY %D 2022 %T Raman Spectroscopy-A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings %A Rebrošová, Katarína %A Samek, Ota %A Kizovský, Martin %A Bernatová, Silvie %A Holá, Veronika %A Růžička, Filip %K BF %K MF %B FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY %V 12 %P 866463 %8 APR 22 %G eng %R 10.3389/fcimb.2022.866463 %0 Journal Article %J ACS Applied Materials & Interfaces %D 2021 %T Optically transportable optofluidic microlasers with liquid crystal cavities tuned by the electric field %A Jonas, Alexandr %A Pilát, Zdeněk %A Ježek, Jan %A Bernatová, Silvie %A Jedlička, Petr %A Aas, Mehdi %A Kiraz, Alper %A Zemánek, Pavel %K BF %K LF %K MF %B ACS Applied Materials & Interfaces %V 13 %P 50657−50667 %8 OCT 21 %G eng %R 10.1021/acsami.1c11936 %0 Journal Article %J NANOPHOTONICS %D 2021 %T The potential of SERS as an AST methodology in clinical settings %A Samek, Ota %A Bernatová, Silvie %A Dohnal, Fadi %K BF %K MF %B NANOPHOTONICS %V 10 %P 2537-2561 %8 AUG %G eng %R 10.1515/nanoph-2021-0095 %0 Journal Article %J Biosensors %D 2021 %T Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris %A Kizovský, Martin %A Pilát, Zdeněk %A Mylenko, Mykola %A Hrouzek, Pavel %A Kuta, Jan %A Skoupý, Radim %A Krzyžánek, Vladislav %A Hrubanová, Kamila %A Adamczyk, Olga %A Ježek, Jan %A Bernatová, Silvie %A Klementová, Tereza %A Gjevik, Alžběta %A Šiler, Martin %A Samek, Ota %A Zemánek, Pavel %K BF %K MF %B Biosensors %V 11 %P 115 %G eng %U https://www.mdpi.com/2079-6374/11/4/115 %R 10.3390/bios11040115 %0 Journal Article %J Future Microbiology %D 2019 %T Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy %A Rebrošová, Katarína %A Šiler, Martin %A Samek, Ota %A Růžička, Filip %A Bernatová, Silvie %A Ježek, Jan %A Zemánek, Pavel %A Holá, Veronika %K BF %K KF %K MF %X

Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: The method showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.

%B Future Microbiology %V 14 %P 509-517 %G eng %U https://doi.org/10.2217/fmb-2018-0297 %R 10.2217/fmb-2018-0297 %0 Journal Article %J J. Phys. Chem. C %D 2019 %T Wavelength-Dependent Optical Force Aggregation of Gold Nanorods for SERS in a Microfluidic Chip %A Bernatová, Silvie %A Donato, Maria Grazia %A Ježek, Jan %A Pilát, Z. %A Samek, Ota %A Magazzu, Alessandro %A Maragò, Onofrio M. %A Zemánek, Pavel %A Gucciardi, Pietro G. %K BF %K MF %B J. Phys. Chem. C %V 123 %P 5608-5615 %G eng %R 10.1021/acs.jpcc.8b12493