TY - JOUR T1 - Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy JF - Future Microbiology Y1 - 2019 A1 - Rebrošová, Katarína A1 - Šiler, Martin A1 - Samek, Ota A1 - Růžička, Filip A1 - Bernatová, Silvie A1 - Ježek, Jan A1 - Zemánek, Pavel A1 - Holá, Veronika KW - BF KW - KF KW - MF AB -

Aim: Finding rapid, reliable diagnostic methods is a big challenge in clinical microbiology. Raman spectroscopy is an optical method used for multiple applications in scientific fields including microbiology. This work reports its potential in identifying biofilm positive strains of Candida parapsilosis and Staphylococcus epidermidis. Materials & methods: We tested 54 S. epidermidis strains (23 biofilm positive, 31 negative) and 51 C. parapsilosis strains (27 biofilm positive, 24 negative) from colonies on Mueller-Hinton agar plates, using Raman spectroscopy. Results: The accuracy was 98.9% for C. parapsilosis and 96.1% for S. epidermidis. Conclusion: The method showed great potential for identifying biofilm positive bacterial and yeast strains. We suggest that Raman spectroscopy might become a useful aid in clinical diagnostics.

VL - 14 UR - https://doi.org/10.2217/fmb-2018-0297 ER -