<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pavel Pořízka</style></author><author><style face="normal" font="default" size="100%">Daniel Holub</style></author><author><style face="normal" font="default" size="100%">Martin Kizovský</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Jozef Kaiser</style></author><author><style face="normal" font="default" size="100%">Gabriela Kalčíková</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Vivek Kumar Singh</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Chapter 26 - Hyphenated Raman and laser spectroscopy for the characterization of microplastics in tissues</style></title><secondary-title><style face="normal" font="default" size="100%">Applied Raman Spectroscopy</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/B9780443218347000268</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Elsevier</style></publisher><pages><style face="normal" font="default" size="100%">441-451</style></pages><isbn><style face="normal" font="default" size="100%">978-0-443-21834-7</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;This chapter is dedicated to the possibility of combining Raman spectroscopy with other spectroscopic methods, namely laser-induced breakdown spectroscopy (LIBS) in the detection and characterization of microplastics embedded in tissues of selected model organisms. The idea is to take advantage of the complementarity of the two methods, which originates in the way of laser-tissue interaction that both techniques utilize for sample analysis. Their joint use yields complete chemical information, that is, Raman spectroscopy provides molecular information, whereas LIBS provides atomic information as is well-described in the literature. Ultimately, the tandem LIBS–Raman analysis can be done from the same spot of the sample. Moreover, these two methods need very similar components and thus can share the majority of the laboratory setup. The chapter gives an overview of hyphenated Raman and LIBS systems, advancements in this field, and options for data processing of joint Raman and LIBS analyses. Data fusion of Raman spectroscopy and LIBS is also discussed. Special attention will be given to the detection and characterization of microplastics using Raman and LIBS systems, as microplastic pollution has attracted considerable attention in recent years, with news of microplastic detection in recent areas and also in human bodies resonating publicly. Raman spectroscopy may be utilized in the detection of microplastics and recent works support this. With the help of LIBS, which can elucidate further the potential risks of weathered microplastics, the hyphenated approach may be the way forward for in situ detection and characterization of microplastics.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Viktória Parobková</style></author><author><style face="normal" font="default" size="100%">Daniel Holub</style></author><author><style face="normal" font="default" size="100%">Martin Kizovský</style></author><author><style face="normal" font="default" size="100%">Gabriela Kalčíková</style></author><author><style face="normal" font="default" size="100%">Ula Rozman</style></author><author><style face="normal" font="default" size="100%">Milan Urík</style></author><author><style face="normal" font="default" size="100%">Karel Novotný</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Tomáš Zikmund</style></author><author><style face="normal" font="default" size="100%">Pavel Pořízka</style></author><author><style face="normal" font="default" size="100%">Jozef Kaiser</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Raman microspectroscopy and laser-induced breakdown spectroscopy for the analysis of polyethylene microplastics in human soft tissues</style></title><secondary-title><style face="normal" font="default" size="100%">Heliyon</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S2405844024138753</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">e37844</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;People are exposed to microplastics (MPs) on a large scale in everyday life. However, it is not clear whether MPs can also be distributed and retained in certain tissues. Therefore, the development of analytical methods capable of detecting MPs in specific human organs/tissues is of utmost importance. In this study, the use and combination of spectroscopic techniques, namely Raman microspectroscopy and laser-induced breakdown spectroscopy (LIBS), was tested for the detection of polyethylene (PE) MPs in human tonsils. Preliminary results showed that Raman microspectroscopy was able to detect MPs down to 1&amp;nbsp;μm in size and LIBS down to 10&amp;nbsp;μm. In the next step, human tonsils were spiked with PE MPs, and digested. The filtered particles were analyzed using Raman microspectroscopy and LIBS, and complemented by X-ray fluorescence (XRF). The results showed that Raman microspectroscopy could reliably detect PE MPs in spiked human tonsils, while LIBS and XRF served as a reference analytical method to characterize particles that could not be classified by Raman microspectroscopy for their non-organic origin. The results of this study, supported by a current feasibility study conducted on clinical samples, demonstrated the reliability and feasibility of this approach for monitoring MPs in biotic samples.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pavel Pořízka</style></author><author><style face="normal" font="default" size="100%">Lukas Brunnbauer</style></author><author><style face="normal" font="default" size="100%">Michaela Porkert</style></author><author><style face="normal" font="default" size="100%">Ula Rozman</style></author><author><style face="normal" font="default" size="100%">Gregor Marolt</style></author><author><style face="normal" font="default" size="100%">Daniel Holub</style></author><author><style face="normal" font="default" size="100%">Martin Kizovský</style></author><author><style face="normal" font="default" size="100%">Markéta Benešová</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Andreas Limbeck</style></author><author><style face="normal" font="default" size="100%">Jozef Kaiser</style></author><author><style face="normal" font="default" size="100%">Gabriela Kalčíková</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Laser-based techniques: Novel tools for the identification and characterization of aged microplastics with developed biofilm</style></title><secondary-title><style face="normal" font="default" size="100%">Chemosphere</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0045653522038668</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">313</style></volume><pages><style face="normal" font="default" size="100%">137373</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Microplastics found in the environment are often covered with a biofilm, which makes their analysis difficult. Therefore, the biofilm is usually removed before analysis, which may affect the microplastic particles or lead to their loss during the procedure. In this work, we used laser-based analytical techniques and evaluated their performance in detecting, characterizing, and classifying pristine and aged microplastics with a developed biofilm. Five types of microplastics from different polymers were selected (polyamide, polyethylene, polyethylene terephthalate, polypropylene, and polyvinyl chloride) and aged under controlled conditions in freshwater and wastewater. The development of biofilm and the changes in the properties of the microplastic were evaluated. The pristine and aged microplastics were characterized by standard methods (e.g., optical and scanning electron microscopy, and Raman spectroscopy), and then laser-induced breakdown spectroscopy (LIBS) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) were used. The results show that LIBS could identify different types of plastics regardless of the ageing and major biotic elements of the biofilm layer. LA-ICP-MS showed a high sensitivity to metals, which can be used as markers for various plastics. In addition, LA-ICP-MS can be employed in studies to monitor the adsorption and desorption (leaching) of metals during the ageing of microplastics. The use of these laser-based analytical techniques was found to be beneficial in the study of environmentally relevant microplastics.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vladimira Tarbajova</style></author><author><style face="normal" font="default" size="100%">Martina Kolackova</style></author><author><style face="normal" font="default" size="100%">Pavel Chaloupsky</style></author><author><style face="normal" font="default" size="100%">Marketa Dobesova</style></author><author><style face="normal" font="default" size="100%">Petr Capal</style></author><author><style face="normal" font="default" size="100%">Zdenek Pilat</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Pavel Zemánek</style></author><author><style face="normal" font="default" size="100%">Pavel Svec</style></author><author><style face="normal" font="default" size="100%">Dagmar Skopalova Sterbova</style></author><author><style face="normal" font="default" size="100%">Marketa Vaculovicova</style></author><author><style face="normal" font="default" size="100%">Lukas Richtera</style></author><author><style face="normal" font="default" size="100%">Alfredo Pérez-de-Mora</style></author><author><style face="normal" font="default" size="100%">Vojtech Adam</style></author><author><style face="normal" font="default" size="100%">Dalibor Huska</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Physiological and transcriptome profiling of Chlorella sorokiniana: A study on azo dye wastewater decolorization</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Hazardous Materials</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0304389423017338</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">460</style></volume><pages><style face="normal" font="default" size="100%">132450</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Over decades, synthetic dyes have become increasingly dominated by azo dyes posing a significant environmental risk due to their toxicity. Microalgae-based systems may offer an alternative for treatment of azo dye effluents to conventional physical-chemical methods. Here, microalgae were tested to decolorize industrial azo dye wastewater (ADW). Chlorella sorokiniana showed the highest decolorization efficiency in a preliminary screening test. Subsequently, the optimization of the experimental design resulted in 70% decolorization in a photobioreactor. Tolerance of this strain was evidenced using multiple approaches (growth and chlorophyll content assays, scanning electron microscopy (SEM), and antioxidant level measurements). Raman microspectroscopy was employed for the quantification of ADW-specific compounds accumulated by the microalgal biomass. Finally, RNA-seq revealed the transcriptome profile of C. sorokiniana exposed to ADW for 72&amp;nbsp;h. Activated DNA repair and primary metabolism provided sufficient energy for microalgal growth to overcome the adverse toxic conditions. Furthermore, several transporter genes, oxidoreductases-, and glycosyltransferases-encoding genes were upregulated to effectively sequestrate and detoxify the ADW. This work demonstrates the potential utilization of C. sorokiniana as a tolerant strain for industrial wastewater treatment, emphasizing the regulation of its molecular mechanisms to cope with unfavorable growth conditions.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Katarína Rebrošová</style></author><author><style face="normal" font="default" size="100%">Silvie Bernatová</style></author><author><style face="normal" font="default" size="100%">Martin Šiler</style></author><author><style face="normal" font="default" size="100%">Jan Mašek</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Jan Ježek</style></author><author><style face="normal" font="default" size="100%">Martin Kizovský</style></author><author><style face="normal" font="default" size="100%">Veronika Holá</style></author><author><style face="normal" font="default" size="100%">Pavel Zemánek</style></author><author><style face="normal" font="default" size="100%">Filip Růžička</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Rapid Identification of Pathogens Causing Bloodstream Infections by Raman Spectroscopy and Raman Tweezers</style></title><secondary-title><style face="normal" font="default" size="100%">Microbiology Spectrum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://journals.asm.org/doi/abs/10.1128/spectrum.00028-23</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">e00028-23</style></pages><language><style face="normal" font="default" size="100%">eng</style></language></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ondřej Vaculík</style></author><author><style face="normal" font="default" size="100%">Silvie Bernatová</style></author><author><style face="normal" font="default" size="100%">Katarína Rebrošová</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Lukáš Šilhan</style></author><author><style face="normal" font="default" size="100%">Filip Rů žička</style></author><author><style face="normal" font="default" size="100%">Mojmír Šerý</style></author><author><style face="normal" font="default" size="100%">Martin Šiler</style></author><author><style face="normal" font="default" size="100%">Jan Jež ek</style></author><author><style face="normal" font="default" size="100%">Pavel Zemánek</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods</style></title><secondary-title><style face="normal" font="default" size="100%">Biomed. Opt. Express</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AIF</style></keyword><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">LF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Dec</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://opg.optica.org/boe/abstract.cfm?URI=boe-14-12-6410</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">6410–6421</style></pages><language><style face="normal" font="default" size="100%">eng</style></language></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Daniel Holub</style></author><author><style face="normal" font="default" size="100%">Pavel Pořízka</style></author><author><style face="normal" font="default" size="100%">Martin Kizovský</style></author><author><style face="normal" font="default" size="100%">David Prochazka</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Josef Kaiser</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The potential of combining laser-induced breakdown spectroscopy and Raman spectroscopy data for the analysis of wood samples</style></title><secondary-title><style face="normal" font="default" size="100%">Spectrochimica Acta Part B: Atomic Spectroscopy</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0584854722001318</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">195</style></volume><pages><style face="normal" font="default" size="100%">106487</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;We report on combining the surface analysis technique of Laser-induced Breakdown Spectroscopy (LIBS) with Raman spectroscopy. The combination of both techniques enables to study the chemical composition of the sample in a broader context when combining elemental and molecular information. Obtained elemental and molecular spectra are characteristic for individual biological samples (e.g., organs and cells) and are considered as a fingerprint. In this study, Raman spectroscopy is used for the detection of important molecular complexes in selected wood samples, e.g., accurate lignin and cellulose content on distinct spots of the sample surface. We chose Raman as a standard reference technique that is used for the lignin/cellulose ratio estimation. To complement the molecular information, LIBS technique was employed for the imaging of essential nutrients, e.g., Ca, Na, and K. Consequently, the contribution from both analytical techniques was combined and changes in the molecular content were visually correlated to the abundance of nutrition elements and show a direct dependence between the two signal responses. Thus, we can get specific answers to relation of lignin and cellulose formation with nutrients within the plant tissue. This evidence may then be helpful for the study of the effect of various environmental and stress factors.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Katarína Rebrošová</style></author><author><style face="normal" font="default" size="100%">Silvie Bernatová</style></author><author><style face="normal" font="default" size="100%">Martin Šiler</style></author><author><style face="normal" font="default" size="100%">Magdalena Uhlirova</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Jan Ježek</style></author><author><style face="normal" font="default" size="100%">Veronika Holá</style></author><author><style face="normal" font="default" size="100%">Filip Růžička</style></author><author><style face="normal" font="default" size="100%">Pavel Zemánek</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Raman spectroscopy—a tool for rapid differentiation among microbes causing urinary tract infections</style></title><secondary-title><style face="normal" font="default" size="100%">Analytica Chimica Acta</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">KF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0003267021011181</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">1191</style></volume><pages><style face="normal" font="default" size="100%">339292</style></pages><language><style face="normal" font="default" size="100%">eng</style></language></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ivana Novackova</style></author><author><style face="normal" font="default" size="100%">Vendula Hrabalova</style></author><author><style face="normal" font="default" size="100%">Eva Slaninova</style></author><author><style face="normal" font="default" size="100%">Petr Sedlacek</style></author><author><style face="normal" font="default" size="100%">Ota Samek</style></author><author><style face="normal" font="default" size="100%">Martin Koller</style></author><author><style face="normal" font="default" size="100%">Vladislav Krzyzanek</style></author><author><style face="normal" font="default" size="100%">Kamila Hrubanova</style></author><author><style face="normal" font="default" size="100%">Katerina Mrazova</style></author><author><style face="normal" font="default" size="100%">Jana Nebesarova</style></author><author><style face="normal" font="default" size="100%">Stanislav Obruca</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Biological Macromolecules</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">BF</style></keyword><keyword><style  face="normal" font="default" size="100%">MF</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0141813022005712</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">206</style></volume><pages><style face="normal" font="default" size="100%">977-989</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Polyhydroxyalkanoates (PHA) are abundant microbial polyesters accumulated in the form of intracellular granules by numerous prokaryotes primarily as storage of carbon and energy. Apart from their storage function, the presence of PHA also enhances the robustness of the microbial cells against various stressors. In this work, we investigated the role of PHA in Cupriavidus necator, a model organism concerning PHA metabolism, for adaptation to osmotic pressure and copper ions. In long-term laboratory evolution experiments, the bacterial culture was cultivated in presence of elevated doses of sodium chloride or copper ions (incubations lasted 78 passages for Cu2+ and 68 passages for NaCl) and the evolved strains were compared with the wild-type strain in terms of growth and PHA production capacity, cell morphology (investigated by various electron microscopy techniques), activities of selected enzymes involved in PHA metabolism and other crucial metabolic pathways, the chemical composition of bacterial biomass (determined by infrared and Raman spectroscopy) and also considering robustness against various stressors. The results confirmed the important role of PHA metabolism for adaptation to both tested stressors.&lt;/p&gt;</style></abstract></record></records></xml>